evaluation of different phenotypic and genotypic methods for detection of methicillin resistant staphylococcus aureus (mrsa)
Authors
abstract
background: methicillin resistant staphylococcus aureus (mrsa) has been emerged as a nosocomial and community acquired pathogen worldwide. there are many challenges for laboratory detection of mrsa. the aim of this study was to compare different phenotypic methods with pcr based method as a gold standard for detection of meca gene. methods: a total of 220 clinical isolates of s. aureus which were isolated from various clinical specimens from september 2013 until the june of 2014 in milad hospital of tehran, iran was subject of our study. methicillin resistance was determined by oxacillin and cefoxitin disks, oxacillin screen agar and chromagar™ mrsa medium. the results of these methods were compared with meca gene based pcr method as a gold standard method. results: among 220 isolates from s. aureus, 105 (47.72%) isolates were positive for meca gene by pcr method. the results of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as pcr method .chromagar™ mrsa medium had 98.13% sensitivity and 100% specificity. oxacillin disk diffusion and oxacillin screen agar had 95.42% and 97.22% sensitivity respectively. conclusion: result of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as pcr method for detection meca gene. cefoxitin disk diffusion method can be used as an alternative method of pcr for detection of mrsa.
similar resources
Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...
full textEvaluation of Five Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
BackgroundandObjectives:RapidandaccuratedetectionofmethicillinresistantStaphylococcus aureus (MRSA) is an important role of clinical microbiology laboratories to avoid treatment failure. The aim of this study was to compare conventional methods against the E-test minimum inhibitory concentration (MIC) method to determine the best phenotypic method. Materials a...
full textEvaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
BACKGROUND Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. METHODS A total of 220 clinical isolates of S. aureus which we...
full textevaluation of five phenotypic methods for detection of methicillin resistant staphylococcus aureus (mrsa)
backgroundandobjectives:rapidandaccuratedetectionofmethicillinresistantstaphylococcus aureus (mrsa) is an important role of clinical microbiology laboratories to avoid treatment failure. the aim of this study was to compare conventional methods against the e-test minimum inhibitory concentration (mic) method to determine the best phenotypic method. materials and methods: methicillin resistance...
full text[Phenotypic methods for detection of methicillin-resistant Staphylococcus aureus].
BACKGROUND Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30 μg) to oxacillin agar screening from detection of the mecA gene by PCR. METHODS Thre...
full textGenotypic and phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis.
The aim of this study was to evaluate the presence of methicillin-resistant Staphylococcus aureus (MRSA) among a (S. aureus) collection (n = 430) isolated from milk of cows suffering from mastitis in Belgium and to compare their genotypic as well as phenotypic characteristics. Pulsed field gel electrophoresis (PFGE) and PCR-based typing techniques (MLST, spa, SCCmec, and agr typing) have been a...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of pathologyجلد ۱۱، شماره ۴، صفحات ۳۷۰-۳۷۶
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023